DNA Extraction Kit (speciation)

Suitable for testing:

The BIOKITS DNA Extraction Kit (Speciation) is intended to be used for the purification of high quality DNA from raw and cooked meats & fish, as well as a wide range of food and feed matrices prior to PCR analysis for the identification of specific species in food and food ingredients, animal feedstuffs etc.

For use with the DNA Animal Speciation Products and DNA Fish Speciation Products

Principle of the Kit:

The BIOKITS DNA Extraction Kit (Speciation) utilises proprietary magnetic bead technology to provide significant advantages over other methods of DNA extraction. The magnetic particles themselves are unique, being specifically designed to bind DNA and not the unwanted matrix components of foods that could interfere with the PCR reaction. The revolutionary combination of the quality and functional properties of the coating used on our particles stands the kit apart from all other systems. One main advantage is that the DNA is not precipitated, therefore co-precipitation of interfering components does not occur. Once the DNA has been immobilised on the surface of the particles they can be washed repeatedly; the resulting quality and purity of the DNA produced speaks for itself.

Unique - proprietary magnetic particles and coating
Simple - no precipitation step
Pure DNA - able to wash particles repeatedly
Versatile - suitable for qualitative and quantitative analysis

All batches of DNA Extraction kits are controlled under our ISO9001 quality system to ensure reproducible, high yields of pure DNA from a wide variety of sample types.

Samples are initially rendered homogeneous, then magnetic particles are used to selectively purify DNA molecules from sample lysates. DNA in the lysate is bound by the beads and the bead/DNA complex is washed with an ethanol solution. DNA is eluted off the magnetic beads using a buffered solution.

Product Details:

Suitable applications:	Qualitative speciation analysis of raw and cooked meats & fish, as well as a wide range of food and feed matrices.
DNA Size Limit:	Minimum of 50 base pairs
Magnetic particles:	Tepnel proprietary technology
Samples tested per kit:	Up to 100 sample extractions
Hands-on time:	< 2 hours (for 10 samples)
Kit Contents:	Extraction solutions (1, 2), DNA Binding solution, Magnetic beads, Proteinase K, Gel Loading Dye
Additional equipment:	Magnetic Rack, Micro-pipettes (20-1000μl), centrifuge, incubator, portable chiller, vortex mixer, balance
Reagent stability:	Several weeks or months after opening
Shelf-life:	Up to 18 months from manufacture
Storage:	With the exception of Proteinase K & Loading Dye, the BIOKITS DNA EXTRACTION KIT (GMO & Allergen) can be stored at ambient temperature. PROTEINASE K reagent MUST BE STORED AT -20°C. Loading Dye should be stored at 2-8°C.

Ordering Information:

BIOKITS DNA EXTRACTION KIT (Speciation) - 901040N.