Qualitative tests for the detection of species content in uncooked foods
| Cow/Pig/Poultry/Sheep |
Cat.No. 902025D |
| Cow |
Cat.No. 902032G (Special Order)
|
| Pig |
Cat.No. 902008D (Special Order) |
| Poultry |
Cat.No. 902035Z (Special Order)
|
| Sheep |
Cat.No. 902034C (Special Order) |
| Other species |
Please enquire |
Intended Use:
These BIOKITS products employ the principles of ELISA to provide sensitive and specific tests and are the most comprehensive range of RAW Species Identification Kits available. These features aid the resolution of species content in uncooked food products by Government and other control laboratories around the world. The identification of animal species is performed for a variety of reasons, both economic and ethnic, to prevent the substitution of meat with unsuitable or inferior species, or in religious communities where a particular meat is proscribed.
Suitable for Testing:
Qualitative detection of Cow, Pig, Poultry and Sheep species in uncooked food products, serum, plasma and dairy products.
The BIOKITS Raw Meat Speciation Kit offers
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Popular Qualitative format
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Detects <1% Cow, Pig, Poultry, Sheep |
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Highly sensitive and specific
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No known ‘ false’ positives |
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BIOKITS validated method
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Reliable results aid analytical decisions |
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Versatility
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Available as single or mixed species |
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Cost Effective
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Colour coded ‘Breakapart’ plates |
Principle of the Assay:
The kits utilise anti-species albumin antibodies coated onto microwells in a direct sandwich type ELISA. Albumin from a sample extract binds to the antibody coated wells. Any unbound material is removed by aspiration and washing. A peroxidase enzyme labelled anti-species antibody then binds to the captured albumin from the samples, to complete the ‘sandwich’. Excess conjugate is removed and the peroxidase activity is determined by the addition of TMB substrate which develops a blue colour. The BIOKITS Raw Meat Species Identification Kit is a sensitive and specific qualitative test for species content in uncooked food products etc.
Methodology:
Food samples are extracted using 0.9% saline solution. The diluted extract is added to the microwells and reacted in three successive 10 minute incubations, interspersed with wash steps, and the addition of peroxidase enzyme labelled anti-species antibody and TMB, respectively. The ELISA results in development of a blue colouration (which turns yellow when ‘stopped’), the level of which is proportional to the amount of albumin present in the sample. A qualitative result can be verified by comparison against the Kit Controls provided.
Assay Schematic:
Product Details:
| Limit of detection |
<1% Cow. Pig, Poultry , Sheep |
| Cross-reactivity |
Comprehensive validation report of uncooked meat / meat product testing (including offal, milk, sera and spike recovery) available on request. |
| Samples tested per kit |
Up to 87 samples single species kits;
Up to 21 samples x 4 different species.
|
| Hands-on time |
Preparation/extraction – five samples, 1 hour |
| Kit Contents |
Full reagent set to perform qualitative analysis, including 96-well coated microwell system with single strip format of 12 x 8 wells;
Controls; Product Insert & Worksheet
|
| Additional equipment |
Micro-pipettes (50-100Hl), wash bottle, (plate reader + 450nm filter optional).
|
| Reagent Stability |
Several weeks or months after opening |
| Shelf-life |
Up to 18 months from manufacture |
| Storage |
2°C to 8°C |
