Robust Single-plex Chemistry
Taqman chemistry
In a Taqman genotyping assay (Applied Biosystems), two probes (each labelled with different fluorophore and quenching groups) are designed such that each is a perfect match to the base variants present at a SNP site. The 5' nuclease activity of a DNA polymerase degrades perfectly matched probes during PCR, liberating the fluorophore from the quencher to give an increase in fluorescence thus revealing the genotype of the sample.
