DNA Fish Speciation Products

BIOKITS DNA PCR products provide a range of testing options for the qualitative detection of Fish Species, at a time when global fish stocks are critically debated and there is rising concern over the authenticity of samples. These latest qualitative tests create new possibilities for testing whether by diligent food processors or for inspection and enforcement.

A choice of eight species can be detected, using a combination of magnetic particles to selectively purify DNA from sample lysates, multiplex PCR reactions and agarose gel detection.

Suitable for testing:

The BIOKITS DNA products enable the specific detection of Cod, Hake, Coley, Haddock, Pollock, Whiting, Trout and Salmon in most types of raw and processed materials.

Principle of the Kit:

The BIOKITS DNA Extraction (Speciation) kit enables sample extraction using Tepnel’s proprietary magnetic bead technology. It is used in combination with the BIOKITS Fish Speciation Selection Module which involves amplification by PCR and detection by agarose gel electrophoresis. The Module contains a choice of species specific PCR mastermixes, packaged as colour-coded “Pods”. These are all-in-one mixes, requiring only the template and Taq polymerase to be added. The mastermixes are pink in colour because they contain gel loading dye which eliminates the need for a procedural step later on. PCR reactions are Multiplex in design. Spike and control DNAs are also included to ensure the reliability of the tests. The agarose gel detection method has been simplified and the combination of high quality extraction and PCR reagents aids the interpretation of

results.

Benefits of tests:

Unique - first commercial kits available for the detection of DNA in fish species
Simple - multiplex PCR reactions to reduce analysis time
Reliable - able to differentiate eight different fish species
Versatile - suitable for qualitative analysis of food and feed

Confidence in BIOKITS DNA products can be assured because firstly all products have been thoroughly tested in the BioGenetix_ partnership of laboratories working with Tepnel and secondly because batches are controlled under the ISO9001 quality system to ensure reproducible and repeatable DNA tests from a variety of sample types.

Product Details:

Suitable applications:	Qualitative identification of fish species in food and feed
Samples tested:	Up to 100 sample extractions per Extraction Kit Up to 300 PCR reactions per Module
Hands-on time:	< 5 hours (for 10 samples) for extraction and PCR
Products Required:	BIOKITS DNA EXTRACTION KIT (Speciation)and BioKits Fish Speciation Selection Module
Shelf-life:	Up to 12 months from manufacture
Storage:	As specified in product insert (requirement for storage at ambient temperatures, at 2-8°C & BELOW -18°C)

Ordering Information:

BIOKITS Fish Speciation Selection Module

200 Tests (Any 2 Pods) 300 Tests (Any 3 Pods)

Catalogue No.

BIOKITS PCR MASTERMIX POD (Cod) 503130G

BIOKITS PCR MASTERMIX POD (Hake) 503131E

BIOKITS PCR MASTERMIX POD (Coley) 503132C

BIOKITS PCR MASTERMIX POD (Haddock) 503133Z

BIOKITS PCR MASTERMIX POD (Pollock) 503134X

BIOKITS PCR MASTERMIX POD (Whiting) 503135V

BIOKITS PCR MASTERMIX POD (Trout) 503136T

BIOKITS PCR MASTERMIX POD (Salmon) 503137R

Additional products required for DNA Extraction are available as follows:

BIOKITS DNA EXTRACTION KIT (Speciation) 901040N

BIOKITS MAGNETIC RACK 901038Y

Each Mastermix Pod above is provided with the corresponding Positive Control Pod Related DNA analytical service: An ISO17025 accredited fish speciation service is offered to suit the needs of all customers.

BIOKITS products are suitable for laboratory use or on-site testing by offering a choice of formats. Other product applications include detection of: Food Allergens, Genetically Modified Ingredients, Animal Speciation and Mycotoxins.