Qualitative tests for the detection of species content in foods/feeds
Beef/Pork/Poultry/Sheep Cat. No. 902011Q 96 well
Pork Cat. No. 902012N 48 well
Beef Cat. No. 902013L 48 well
Poultry Cat. No. 902023H 48 well
Sheep Cat. No. 902022K 48 well
Intended Use:
The identification of species content in food/animal feeds is an important control measure used to detect contamination with, or substitution of, inferior or prohibited animal species into products containing animal-derived materials. The market leading BIOKITS range of (Cooked) Species Identification Kits are used by many food industry laboratories and Government Food Control agencies around the world.
Suitable for:
qualitative detection of species content in cooked/canned foods, rendered animal feeds etc.
The BIOKITS range of (Cooked) Species Identification Kits offers:
- Sensitive and specific Qualitative format
- Superior performance Detection limit <1% beef/pork/poultry; <2% sheep)
- even in highly-treated/autoclaved samples
- Widely recognised method Based on “Berger et al” USDA protocol
- BioKits validated method Reliable results aid analytical decisions
- Versatility Applicable to cooked/canned/rendered food/feed
- Simplicity Testing requires micropipette & wash bottle only
Principle of the Assay:
The kit utilises antibodies to heat-stable, species-specific muscle proteins, coated onto micro-wells, in a non-competitive, sandwich type EIA. Specific proteins from a sample extract bind to the antibody coated wells. A biotinylated anti-species antibody then binds to the captured protein from the samples, to complete the ‘sandwich’. The presence of the biotinylated antibody is then detected using avidin peroxidase (HRP) reagent with TMB as substrate. The BIOKITS (Cooked) Species Identification Kits are sensitive and specific qualitative tests for animal species content in the presence of other food proteins.
Methodology:
Food/feed samples are extracted using a simple saline solution. The extract is added to the microwells and reacted in successive 45, 45, 15, 45 minute incubations, interspersed with wash steps, with biotinylated anti-species antibody, Avidin-HRP and TMB, respectively. The ELISA results in development of a blue colour, the level of which is proportional to the amount of species content in the sample. A qualitative analysis can be obtained by comparison against the Positive and Negative Kit Controls provided, either by eye or using a plate reader or visible spectrophotometer.
Product Details:
Limit of Detection <1% (beef, pork, poultry); <2% (sheep)
Cross-reactivity Beef: reacts with beef, buffalo; variable reaction to dairy products
Pork: reacts only to porcine species
Poultry: reacts with a wide variety of avian species; variable reaction to eggs
Sheep: reacts to sheep, goat.
Samples tested per kit Up to 45 samples (single species 48 well kits); 21 samples (4 species 96 well kits respectively)
Hands-on time Preparation/extraction – five samples, <1 hour
Kit Contents Full reagent set to perform Qualitative analysis, including 96- & 48-well
coated microwell system with single strip format of 8 wells; Positive and
Negative Controls; product insert & worksheet.
Additional equipment Micro-pipettes (50-100El), wash bottle, (plate reader with 450nm filter required for calculated cut-off determinations).
Reagent Stability Several weeks or months after opening
Shelf-life Up to 18 months from manufacture
Storage 2°C to 8°C
Products in the BioKits range are suitable for laboratory or on-site testing.
Two formats based on immunoassay and DNA technology are available.
Product applications include detection of:
Food Allergens, Genetically Modified Ingredients, Animal Speciation and Mycotoxins.
